FGSC #241

Mutant Type

Genus: N

reporting_genes: lys-2 pk ad-7;ylo-1

species: Neurospora crassa

allele: 37101 C-1810-1 44411;Y30539y

stock: 74

glasgow:

mutagen:

Depositor: WNS

Link Group: VR VR VR;VIL

MT: A

Species No: 10

gene_back:

oppmt: 0

trans:

ref1:

ref2:

site:

country:

ksudc_link: https://digital.lib.k-state.edu/item/neurospora-crassa/fgsc-241

ksudc_link_html: https://digital.lib.k-state.edu/item/neurospora-crassa/fgsc-241 ↗

Genes

Locus	Cultural Requirements	Link Group	Type
ad-7	VR. Right of cot-2 (4%). Left of ro-4 (4%) and pab-2(8%). (158, 156). (687) Uses adenine or hypoxanthine (682). Lacks phosphoribosylpyrophosphate amidotransferase, the first enzyme in de novo purine biosynthesis (525) (Fig. 8). Ascospores from homozygous ad-7 x ad-7 crosses are white (allele Y175M256). Strains carrying allele P73Bl7l(t) are temperature sensitive.	VR	B
lys-2	VR. Right of ilv-1 (4 to 7%). Left of cyh-2 (<1%) and leu-5 (9%) (3, 818, 839). (399)Requires lysine. Will not use epsilon-hydroxynorleucine (400). Probably blocked in conversion of alpha-aminoadipate semialdehyde to saccharopine (1087) (Fig. 16). Initial allele: 37101.	VR	B
pk	bis, biscuit) VR. Between met-3 (1%) and T(EB4)^L, cot-2 (8%), cl (2%). Growth on an agar surface is initially colonial and flat. A mass of aerial hyphae is then sent up, which conidiates profusely (1548). Somewhat similar in morphology to sn, cum, sp, and cot-4 at 25ºC, but distinguishable. Hyphae branch dichotomously (1405, 1548). Increased activity of L-glutamine:D-fructose-6-phosphate amidotransferase was observed in crude extracts of one pk strain, but not in nine others; increased activity for this enzyme also was found in cl and in four other nonallelic morphological mutants (1758). Hexoseaminoglycan consists of a single component on medium without sorbose, in contrast to two components in wild type (1960). Antigenic surface mucopolyoside (532). Cell-wall analysis and photograph, allele B6 (507) and allele C-1810-1 (287). Cell-wall enzymes (633). Effect of carbon source (531). One observation suggested a functional interaction with cl (1965), but substantial crossing-over frequencies and recovery of the double mutant pk cl indicated that the loci are distinct (569). The gene was named for the vegetative mutant phenotype, which is recessive. Several alleles were called bis (1592). The sexual phase is also affected. Asci are thin-walled, bulbous, and nonlinear in homozygous pk ´ pk crosses (1406, 1409, 1550). Spindle orientation is abnormal in the swollen asci, and no apical pore is formed (1625, 1679). Most mutant alleles are recessive for the ascus effect, but some are dominant with variable penetrance. Sorbose-resistant mutants at various loci act as dominance modifiers of the ascus effect of dominant alleles (1757). Sexual-phase-recessive allele C-1610 and dominant allele 17-088 are both associated with reciprocal translocations (1578).		B
ylo-1	VIL. Between cys-1 (8%) and ad-1 (6%). Probably right of Bml (2%) (1012, PB). (381). Yellow carotenoids (381). Affects synthesis of neurosporaxanthin (4'-apo-beta'-caroten-4'-oic acid); citations in reference 398. Lesion probably involves the conversion of lycopene to 3,4-dehydrolycopene or the conversion of either torulene or gamma-carotene to neurosporaxanthin (398 and references therein) (Fig. 9). Resembles the orange wild type in young cultures, but color differences become clear with age. Expressed in both conidia and mycelia. Undefined modifiers affect intensity. Fails to complement with many of the al-1 and al-2 albino strains (R.E. Subden, personal communication).	VIL	B