Strain: Neurospora crassa

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FGSC #263

Mutant Type

Genus: N

reporting_genes: al-2 cnr

species: Neurospora crassa

allele: 15300 no#(s)

stock: 1260?

glasgow:

mutagen:

Depositor: DDP

Link Group: IR

MT: a

Species No: 10

gene_back:

oppmt: 0

trans:

ref1:

ref2:

site:

country:

ksudc_link: https://digital.lib.k-state.edu/item/neurospora-crassa/fgsc-263

ksudc_link_html: https://digital.lib.k-state.edu/item/neurospora-crassa/fgsc-263 ↗

Genes

Locus Cultural Requirements Link Group Type
cnrIR. Linked to hom (1%) and nic-1 (11%), probably between them (812). (1061) Resistance to canavanine (480, 1061, 1062) is due to a constitutive enzyme that cleaves L-canavanine to hydroxyguanidine plus a compound which can be converted to L-homoserine (617). Resistance is altered secondarily by modifiers that affect rate of uptake (617). Many laboratory strains are resistant, but a few are sensitive (790). Best scored on 0.2 mg of L-canavanine H2SO4 per ml (autoclaved in the medium) at 2 days, 34°C (812; D.D. Perkins, unpublished data). Sensitive strain used to select resistant mutants defective in basic amino acid transport (889, 913, 1152); see pmb. Called can.IRB
al-2IR. Right of os-5 (<1%) and T(STL76). Left of arg-6(1%) and al-1 (797, 802, 808, 816, 818). Included in duplications from Tp(T54M94), confirming location left of arg-6(808). (482) Carotenoids absent or abnormal, but steroids produced (398). Blocked in microsomal fraction and defective in phytoene synthetase (445), a particulate enzyme (445 and references cited therein) (Fig. 9). Tracer experiments indicate a lesion between prephytoene pyrophosphate and phytoene (572). Alleles include those resulting in white and pale rose-white, e.g., 15300 and Y254MI65 (1042), and purple, e.g., MN58a (154). For complementation, see references 500 and 1041. Fine-structure mapping (500, 1042) needs reevaluation because of new information on the location of the arg-6 marker (797).IRB

Neurospora Crassa Wikipedia

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