FGSC #1701

Mutant Type

Genus: N

reporting_genes: ota

species: Neurospora crassa

allele: UM728

stock: otab3R1a(UM-

glasgow:

mutagen: UV

Depositor: RHD

Link Group: IIIR

MT: a

Species No: 10

gene_back: SL3

oppmt: 1700

trans:

ref1: Davis & Mora J Bact 96:383-388 1968 XD16, https://doi.org/10.1128/jb.96.2.383-388.1968

ref2:

site:

country:

ksudc_link: https://digital.lib.k-state.edu/item/neurospora-crassa/fgsc-1701

Locus	Cultural Requirements	Link Group	Type
ota	IIIR. Between ad-4 (15%) and tyr-1 (14%) (241). Linked to pro-4 (4%) (D.J. West, cited in Neurospora Newsl. 16:19-22, 1970). Ornithine-delta-transaminase deficient (241) (Fig. 10). Conidiates somewhat less than does the wild type (S. Brody, personal communication). Selected by ability to use exogenous ornithine as a precursor for arginine in an arg-5 arg-12s double mutant. Catabolism of ornithine (to glutamic-gamma-semialdehyde) is blocked, resulting in ornithine concentrations high enough to compensate for the low activity of the ornithine carbamyl transferase in the arg-12s mutant. The ota single mutant is prototrophic but prevents the efficient use of ornithine or arginine as the sole nitrogen source (241). Used to study flux through the arginine biosynthetic pathway (401). Used to study the utilization of endogenous versus exogenous ornithine (234). Sideramine production is completely blocked in absence of ornithine in the ota;arg-5;aga triple mutant, which is used to study iron transport (1146, 1147).	IIIR	B

Neurospora Crassa Wikipedia