FGSC #2178

Mutant Type

Genus: N

reporting_genes: un-5 al-1 R

species: Neurospora crassa

allele: b39(t) ALS4 35408R

stock: 1557

glasgow:

mutagen:

Depositor: DDP

Link Group: IL IR IR

MT: a

Species No: 10

gene_back:

oppmt: 2177

trans:

ref1:

ref2:

site:

country:

ksudc_link: https://digital.lib.k-state.edu/item/neurospora-crassa/fgsc-2178

ksudc_link_html: https://digital.lib.k-state.edu/item/neurospora-crassa/fgsc-2178 ↗

Genes

Locus	Cultural Requirements	Link Group	Type
al-1	IR. Right of hom (<1%), arg-6 (<1 to 4%), T(T54M94), and al-2. Left of lys-3 (9%). (797, 808; D.D. Perkins, unpublished data). (482) Carotenoids abnormal. Strains carrying the various alleles differ widely in phenotype, ranging from white (e.g., 4637) and "aurescent" (pigment in peripheral conidia and conidiophores, 34508) to yellow mycelia and conidia (e.g., ALS4 and RES-25). See, for example, reference 1042. Strains carrying alleles ALS-14, RES-6, 34508, and RES-25 contain large amounts of phytoene (99 to 100% of the total neutral carotenoids), suggesting a lesion that affects phytoene dehydrogenase (398, 1039) (see Fig. 9). Strains carrying allele RWT-ylo accumulate zeta carotene and smaller amounts of neurosporene, suggesting a leaky block of the step between these intermediates (1071). It is not known whether phytoene dehydrogenase catalyzes the whole series of dehydrogenations or whether leakiness of this enzyme accounts for the different mutant phenotypes. For complementation tests, see references 500, 1039, and 1041. Fine-structure mapping (500, 1042). Translocation T(4637), inseparable from al-1, was the first albino mutation and one of the first chromosome rearrangements in Neurospora to be identified and studied (656). Allele 34508 called aur: aurescent.	IR	B
un-5	IL. Right of fr (6%). Left of T(39311) and nit-2 (2%) (816, 798). (574). Unknown function. Heat sensitive, 34°C versus 23°C. Inhibited by histidine and tryptophan: osmophilic (26 to 28 C) (574, 575). Formerly called un(b39).	IL	B
R	IR. Right of T(NM169d) and aro-8 (4%). Left of un-18(11%) (1093). (H.R. Cameron, personal communication) All eight ascospores of heterozygous R/+ asci are round rather than ellipsoid. R is thus nonautonomous in ascospores and dominant in the ascus (690). Ascospores are round even in nonlinear asci (1008; D.D. Perkins, unpublished data). Usually two germination pores are formed, but sometimes one (1008). Photograph (1008). Vegetative morphology abnormal, somewhat resembling that of pe mutants. Initial growth on slants is concentrated around the inoculation point. The vegetative morphology is recessive in heterozygous duplications, as from T(NM103) (1091). Female sterile, with no perithecia, but R x R crosses can be accomplished if R is heterokaryotic in the female parent (A.M. Srb, personal communication). Used in studies of duplication instability (1091) and autonomy of ascospore development (529). Allelic recurrences obtained (1008; A.M. Srb, personal communication). For other genetically determined round ascospores, see reference 59. Called Rsp (1008), but the original symbol R has priority. (rsp has been used for cytoplasmically determined respiration-deficient mutants [890].)	IR	B