FGSC #2713

Mutant Type

Genus: N

reporting_genes: (fz;sg;arg-1 cr-1 al-1 os-1 A + tol pan-1 a)

species: Neurospora crassa

allele: (no#;no# B369 B123 34508 B135) + (N83 5531)

stock: H2 A/a

glasgow:

mutagen:

Depositor: REN

Link Group: --;--;IL IR IR IR + IVR IVR

MT: B

Species No: 10

gene_back:

oppmt: 0

trans:

ref1: Nelson et al 1975 N.N. 22:15-16, https://doi.org/10.4148/1941-4765.1789

ref2:

site:

country:

ksudc_link: https://digital.lib.k-state.edu/item/neurospora-crassa/fgsc-2713

ksudc_link_html: https://digital.lib.k-state.edu/item/neurospora-crassa/fgsc-2713 ↗

Genes

Locus	Cultural Requirements	Link Group	Type
pan-1	IVR. Between ad-6 (1 to 2%) and cot-1 (2 to 3%) (633, 692, PB). (482). cel, col-1, int, pho-3, and thi-5 all appear to be closely linked in this crowded region. Requires intact pantothenic acid for growth under standard conditions. Able to synthesize both precursors, beta-alanine and pantoyl lactone (1058). Ability to synthesize pantothenic acid from beta-alanine plus pantoyl lactone is demonstrable in vitro but not in vivo unless cultures are aerated (1111, 1113, 1114). Unlike pan-2, pan-1 has no effect on ascospore ripening in heterozygous crosses. Called group A. For alleles see reference 138.	IVR	B
A			B
a			B
tol	IVR. Linked to trp-4 (~1%), probably to the left (755). Suppresses the vegetative (heterokaryon) incompatibility associated with mating type alleles A and a, but does not affect sexual compatibility. (tol;A+ tol;a) heterokaryons are fully compatible and stable if other het loci are homokaryotic, and A/a duplications grow normally when tol is present (755). Recessive (252); see reference 746, however, for a stable mixed-mating type heterokaryon that is (tol a + tol+ slime A). tol does not suppress the vegetative incompatibility of differing alleles at het-c or het-e(755, 803). Mutation or deletion of tol+ restores normal growth rate to slow-growing, unstable, mixed-mating-type (tol a + tol+ A) heterokaryons (252). tol is present in some isolates from nature and has arisen at least twice by mutation in laboratory stocks (755, PB; O.C. Yoder, personal communication). Double-mutant tol trp-4 stocks are convenient because the closely linked trp-4 tags the tol allele, which otherwise requires progeny tests for scoring. Used to maintain stable A + a heterokaryons, allowing the desired component to be used as the parent in a cross (746). Homozygous tolmay partially restore fertility to the mutant fmf-1 (531).	IVR	B
sg	Unmapped. Ascospores germinate without heat shock. Usually associated with a very poor vegetative growth habit. A component of the multiply mutant combination resulting in the cell-wall-less "slime" phenotype. Possibly more complex than a single gene (321). See slime.		B
fz	Unmapped.Abnormal morphology; one component of the combination of mutant genes that results in the cell-wall-less "slime" phenotype (321).		B
cr-1	IR. Right of ace-7 (1 to 3%) and nic-2 (4 to 7%). Left of cys-9 (3%) and un-1(5%) (721, 816). Included in duplications from T(4540), which do not include cr-2 or cr-3(PB). (610) Rapid conidiation close to surface of agar. Produces very short conidiophores, bearing conidia in tight clusters (610, 611). Photographs (533, 634). Recessive. Deficient in adenylate cyclase (1066); has little or no endogenous adenosine 3',5'-phosphate (1065, 779). Abnormal morphology partially corrected by exogenous adenosine 3',5'-phosphate (891, 892, 1065, 1066). Guanosine 3',5'-phosphate also stimulates mycelial elongation (892). Cyclic nucteotide levels differ in mycelia and conidia (891, 892). NAD(P) glycohydrolase is overproduced and excreted; this is normalized by adenosine 3',5'-phosphate (533). Induction and localization of p-glucosidase is altered; induction is normalized by adenosine 3',5'-phosphate (906). Inability to use glycerol and certain other carbon sources is also overcome by adenosine 3',5'phosphate (598, 1067). Phosphodiesterase inhibitors do not counteract the morphological effect of cr-1 (892). Increased lactate dehydrogenase activity (92). Used to determine what functions are controlled by adenosine 3',5'-phosphate (779). Used to study adenosine 3',5'-phosphate binding protein (1082). Strains carrying the various alleles vary in growth habit (B123 strains are flat, restricted; allele L strains are spreading, but morphology may vary on different media). Modifier mutations which alter morphology and the ability of cr-1 to use glycerol occur frequently (383, 905). Crosses homozygous for allele B123 exude intact linear asci (634). Double mutants sn cr and cr rg form small conidiating colonies suitable for replica plating with velvet (182, 634, 796, 932, 1020). The triple mutant sn cr;csp-2 can be overlayered (744; photograph 747). The single mutant (B123) can be replicated by using a needle replicator (634). Scorability and viability are good. Excellent as a marker. Carotenoids formed normally. cr-1 ascospores may require longer to mature than cr+ ascospores. Allele CE4-11-67 called con(716, 717).	IR	B
al-1	IR. Right of hom (<1%), arg-6 (<1 to 4%), T(T54M94), and al-2. Left of lys-3 (9%). (797, 808; D.D. Perkins, unpublished data). (482) Carotenoids abnormal. Strains carrying the various alleles differ widely in phenotype, ranging from white (e.g., 4637) and "aurescent" (pigment in peripheral conidia and conidiophores, 34508) to yellow mycelia and conidia (e.g., ALS4 and RES-25). See, for example, reference 1042. Strains carrying alleles ALS-14, RES-6, 34508, and RES-25 contain large amounts of phytoene (99 to 100% of the total neutral carotenoids), suggesting a lesion that affects phytoene dehydrogenase (398, 1039) (see Fig. 9). Strains carrying allele RWT-ylo accumulate zeta carotene and smaller amounts of neurosporene, suggesting a leaky block of the step between these intermediates (1071). It is not known whether phytoene dehydrogenase catalyzes the whole series of dehydrogenations or whether leakiness of this enzyme accounts for the different mutant phenotypes. For complementation tests, see references 500, 1039, and 1041. Fine-structure mapping (500, 1042). Translocation T(4637), inseparable from al-1, was the first albino mutation and one of the first chromosome rearrangements in Neurospora to be identified and studied (656). Allele 34508 called aur: aurescent.	IR	B
arg-1	Uses arginine but not precursors.	IL	B
os-1	IR. Between nic-1 (10 to 29%) and arg-13 (1%) (789, 812, 816). (M.R. Emerson, cited in reference 789) Sensitive to high osmotic pressure. Readily scored by morphology on nonmoist slants or by failure to grow on media with 4% NaCl. Most os-1 alleles result in cultures that form no or few conidia on agar slants. Alleles NM233t and NM204t are heat sensitive (25°C versus 34°C). In media of high osmolarity, os-1 strains form protoplasts (323, 438). os-1 (Bl35) is an essential genotypic component of the wall-less strain slime (321). Protoplasts of strains carrying heatsensitive allele NM233t are stable at 37 C, with a 7.5-h redoubling time, and show good regeneration. The biochemical defect differs from that affected by either polyoxin or sorbose (chitin or glucan syntheses) (970, 971). Cell wall pores are four times larger in an os-1 mutant than in the wild type; os-1 also has a higher exclusion threshold and a 30-fold-higher galactosamine/ glucosamine ratio (1083, 1084). Intralocus complementation (676). Allele Y256M209 called flm-1.	IR	B