Gene: osmotic-1 IR

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osmotic-1

locus: os-1

locus_name: osmotic-1

organism_type: B

chromosome_number: I

chromosome_side: R

link_group: IR

cultural_requirements: IR. Between nic-1 (10 to 29%) and arg-13 (1%) (789, 812, 816). (M.R. Emerson, cited in reference 789) Sensitive to high osmotic pressure. Readily scored by morphology on nonmoist slants or by failure to grow on media with 4% NaCl. Most os-1 alleles result in cultures that form no or few conidia on agar slants. Alleles NM233t and NM204t are heat sensitive (25°C versus 34°C). In media of high osmolarity, os-1 strains form protoplasts (323, 438). os-1 (Bl35) is an essential genotypic component of the wall-less strain slime (321). Protoplasts of strains carrying heatsensitive allele NM233t are stable at 37 C, with a 7.5-h redoubling time, and show good regeneration. The biochemical defect differs from that affected by either polyoxin or sorbose (chitin or glucan syntheses) (970, 971). Cell wall pores are four times larger in an os-1 mutant than in the wild type; os-1 also has a higher exclusion threshold and a 30-fold-higher galactosamine/ glucosamine ratio (1083, 1084). Intralocus complementation (676). Allele Y256M209 called flm-1.

enzyme_id:

anid_name:

near_l: nic-1 (10 to 29%)

near_r: arg-13 (1%)

neu_name:

supplement:

image_url:

Strains

FGSC # Reporting Genes Species
FGSC #34os-1Neurospora crassa
FGSC #47os-1Neurospora crassa
FGSC #228nit-1 al-1 nic-1 os-1Neurospora crassa
FGSC #229Has been replaced by strain number: 3603Neurospora crassa
FGSC #236cr-1 thi-1 nit-1 al-1 nic-1 os-1;cot-1Neurospora crassa
FGSC #245cr-1 thi-1 nit-1 al-1 nic-1 os-1Neurospora crassa
FGSC #250cr-1 al-1 os-1Neurospora crassa
FGSC #258cr-1 nit-1 al-1 os-1Neurospora crassa
FGSC #280cr-1 os-1Neurospora crassa
FGSC #288cr-1 nit-1 al-1 os-1Neurospora crassa
FGSC #289cr-1 os-1Neurospora crassa
FGSC #327(fz;sg;arg-1 cr-1 al1 os-1+ al2 nic-1 lys-3 os-1)Neurospora crassa
FGSC #329arg-1 cr-1 al-1 os-1Neurospora crassa
FGSC #810os-1Neurospora crassa
FGSC #812os-1Neurospora crassa
FGSC #813os-1Neurospora crassa
FGSC #824os-1Neurospora crassa
FGSC #866os-1 al-2Neurospora crassa
FGSC #916Has been replaced by strain number: 3625Neurospora crassa
FGSC #951os-1Neurospora crassa
FGSC #973os-1Neurospora crassa
FGSC #1118Not available; See 1119, 327, 2713; fz;sg;os-1Neurospora crassa
FGSC #1119(fz;sg;os-1) + (arg-1 cr-1 al-1 os-1)Neurospora crassa
FGSC #1200Has been replaced by strain number: 4494Neurospora crassa
FGSC #1287Has been replaced by strain number: 4493Neurospora crassa
FGSC #1508os-1Neurospora crassa
FGSC #1637os-1Neurospora crassa
FGSC #1675os-1Neurospora crassa
FGSC #2273os-1Neurospora crassa
FGSC #2432os-1Neurospora crassa
FGSC #2584os-1Neurospora crassa
FGSC #2713(fz;sg;arg-1 cr-1 al-1 os-1 A + tol pan-1 a)Neurospora crassa
FGSC #3603al-1 nic-1 os-1Neurospora crassa
FGSC #3624os-1Neurospora crassa
FGSC #3625os-1Neurospora crassa
FGSC #3901os-1 (171-1)Neurospora crassa
FGSC #4493os-1Neurospora crassa
FGSC #4494os-1Neurospora crassa
FGSC #4761fz;sg;os-1Neurospora crassa

References

Authors Title Year
Emerson, S.Slime a plasmodoid variant in Neurospora crassa.1963
Emerson, S. , and M. R. Emerson.Production, reproduction and reversion of protoplast-like structures in the osmotic strain ofNeurospora crassa.1958
Hamilton, J. G. , and J. Calvet.Production of protoplasts in an osmotic mutant of Neurospora crassa without added enzyme.1964
Mishra, N. C.Characterization of the new osmotic mutants (os) which originated during genetic transformation in Neurosporacrassa.1977
Perkins, D. D.New markers and multiple point linkage data in Neurospora.1959
Perkins, D. D. , M. Glassey, and B. A. Bloom.New data on markers and rearrangements in Neurospora.1962
Perkins, D. D. , D. Newmeyer, C. W. Taylor, and D. C. Bennett.New markers and map sequences in Neurospora crassa, with adescription of mapping by duplication coverage, and of multiple translocation stocks for testing linkage.1969
Selitrennikoff, C. P.Temperature sensitive protoplast forming osmotic strain of Neurospora -- a few tricks.1982
Selitrennikoff, C. P. , B. L. Lilley, and R. Zucker.Formation and regeneration of protoplasts derived from a temperature-sensitiveosmotic strain of Neurospora crassa.1981
Trevithick, J. R. , and R. L. Metzenberg.Molecular sieving by Neurospora cell walls during secretion of invertase isozymes.1966
Trevithick, J. R. , and R. L. Metzenbeg.Genetic alteration of pore size and other properties of the Neurospora cell wall.1966

Neurospora Crassa Wikipedia

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