FGSC #3483

Mutant Type

Genus: N

reporting_genes: oli;csp-1;bd

species: Neurospora crassa

allele: no#(r);UCLA37 no#

stock: 2428

glasgow:

mutagen:

Depositor: SB

Link Group: VIIR;IL;IVR

MT: A

Species No: 10

gene_back: M

oppmt: 0

trans:

ref1: Dieckmann & Brody 1980 Science 207:896-8, https://doi.org/10.1126/science.6444467

ref2:

site:

country:

ksudc_link: https://digital.lib.k-state.edu/item/neurospora-crassa/fgsc-3483

ksudc_link_html: https://digital.lib.k-state.edu/item/neurospora-crassa/fgsc-3483 ↗

Genes

Locus	Cultural Requirements	Link Group	Type
bd	Because dense bands of conidia are produced on appropriate solid medium at intervals of about 24 hr (1792, 1794), the mutant has been used extensively to study circadian rhythms (239, 560, 621, 1382, 1794). bd has no effect on the underlying clock mechanism, but allows the visible expression of rhythm (622). Grow rate is about 70% that of the wild type (232). Conidiation is enhanced, even on slants (232). CO2 inhibits conidiation and, thus, inhibits banding; bd is much less sensitive than wild type to this effect of CO2 (1792). Biotin starvation leads to a phenocopy in wild type and to increased persistence of banding in bd (2206). Originally identified in a bd; inv strain called "timex" (1791). bd alone is sufficient to cause banding (1794). Used to study conidiation under nonstarvation conditions (1820). Used in a study of morphological differentiation patterns such as concentric rings and radial zonations (528). Expression is affected by changing the concentrations of agar, sugar, and salts. Conidial scatter is eliminated in the double mutant bd; csp (239). Conveniently scored by conidial banding on agar in long tubes or large plates at 25oC in constant dark or in a dark-light cycle, but not in constant light (1791).	IVR	B
csp-1	IL. Between arg-3 (1%) and the T(39311) right breakpoint (972, PB). Conidia fail to separate and become airborne. Photograph (972). Recessive. Cultures on agar readily scored by the "tap test." In water, conidia are freed at 1/10 the wild-type concentration (972). Used in connection with bd for study of circadian rhythms (e.g., reference 114). Useful in student laboratories to avoid contamination (966). Carotenoids tend to be yellowish in young cultures (PB).	IL	B
oli	VIIR. Between met-9 (8 to 24%) and arg-11 (3 to 16%) (960). Linked to frq-1 (<2%) and possibly allelic (282, 283). (959) Resistant to oligomycin. Defective in energy transduction (313). Structural gene for dicyclohexylcarbodiimide-binding proteolipid (subunit 9) of F0 portion of mitochondrial adenosine triphosphate synthetase (958). The amino acid sequence (81 residues) (959) shows extensive homology with the corresponding proteolipid in yeast, in which, in contrast to Neurospora, it is the product of a mitochondrial gene (960, 1109). Specific single amino acid substitutions have been identified for three mutants (959). oli mutants are selected effectively by using double mutant azs; has (called ANT-1), which is deficient in both salicyl hydroxamic acid-sensitive and azide-sensitive alternate oxidase pathways. Scored on 5 µg of oligomycin per ml of liquid medium, 3 days, 30°C (312). Altered period of circadian rhythm cosegregates and reverts with oli (282, 283).	VIIR	B