FGSC #4477

Mutant Type

Genus: N

reporting_genes: un-2;thi-4;nic-3

species: Neurospora crassa

allele: see 4488

stock:

glasgow:

mutagen:

Depositor: RLM

Link Group:

MT: a

Species No: 10

gene_back:

oppmt: 0

trans:

ref1: Metzenberg, et al NN 31:35, https://doi.org/10.4148/1941-4765.1608

ref2:

site:

country:

ksudc_link: https://digital.lib.k-state.edu/item/neurospora-crassa/fgsc-4477

ksudc_link_html: https://digital.lib.k-state.edu/item/neurospora-crassa/fgsc-4477 ↗

Genes

Locus	Cultural Requirements	Link Group	Type
un-2	IR. Between the T(AR173) left breakpoint and T(AR190); hence, right of the centromere and arg-3 (1 to 2%) and left of his-2 (1%). Included in duplications from T(AR173) but not from T(AR190) (808). (482). Unknown function. Heat sensitive (482). Growth at 25°C but not at 39°C. Scorable but leaky at 34°C. Formerly called un(46006).	IR	B
thi-4	III. Linked to acr-2, spg, and sc (< 1%). Left of pro-1(3%) and ace-2 (4 to 9%) (812). (482). Requires thiamine (301). Anomalous in condensation of the pyrimidine and thiazole precursors. Very leaky (301). A probable allele called "thi-lo" greatly increases the thiamine requirement of thi-1 strains and decreases the ability to synthesize thiamine from pyrimidine and thiazole. thi-lo strains have no detectable nutritional requirement in the absence of thi-1. thi-lo failed to recombine with thi-4 among 55 scorable progeny (301).	III	B
nic-3	VIIL. Right of spco-4 (1%) and do (3%). Left of thi-3 (9 to 27%) and csp-2 (16 to 22%) (539, 812, 816, PB). (M.K. Allen, cited in references 718 and 789) Uses nicotinic acid, nicotinamide, 3-hydroxyanthranilic acid, 3-hydroxykynurenine, or high concentrations of quinolinic acid (96, 1168). Accumulates alpha-N-acetylkynurenine; blocked in conversion of kynurenine to 3-hydroxykynurenine (1168) (Fig. 18). Pyridine nucleotide levels (111). Pathway from tryptophan to nicotinic mononucleotide, showing sites of gene action (96, 100, 368, 1168). The enzymatic reactions between 3-hydroxyanthranilate and nicotinic mononucleotide have not been demonstrated directly in Neurospora.	VIIL	B