FGSC #5062

Mutant Type

Genus: N

reporting_genes: cyt-18 al-2

species: Neurospora crassa

allele: 299-9 ?

stock: 299-9

glasgow:

mutagen: UV

Depositor: AL

Link Group: IR R

MT: a

Species No: 10

gene_back: SL

oppmt: 0

trans:

ref1: Pittenger and West, 1979. Genetics 93:539-555, https://doi.org/10.1093/genetics/93.3.539

ref2: Collins and Lambowitz 1985. J. Mol. Biol. 184:413, https://doi.org/10.1016/0022-2836(85)90291-8

site:

country:

ksudc_link: https://digital.lib.k-state.edu/item/neurospora-crassa/fgsc-5062

Locus	Cultural Requirements	Link Group	Type
cyt-18	IR. Linked to al-2 (10%) and nic-1 (1 to 5%) (203, 635).Heat sensitive; grows slowly. Deficient in cytochromes aa3 and b at 37°C (832). Mitochondrial protein synthesis and assembly of small mitochondrial subunits are also abnormal (205). A novel large RNA precursor (35S) is found at restrictive temperatures (205); the intervening sequence of large (25S) mitochondrial rRNA is apparently not excised. Two alleles (289-67, 299-9) differ in speed of turn-off of RNA processing when the temperature is shifted (635). Complements cyt-4 and cyt-19, which are also required for splicing of large mitochondrial rRNA (83).	IR	B
al-2	IR. Right of os-5 (<1%) and T(STL76). Left of arg-6(1%) and al-1 (797, 802, 808, 816, 818). Included in duplications from Tp(T54M94), confirming location left of arg-6(808). (482) Carotenoids absent or abnormal, but steroids produced (398). Blocked in microsomal fraction and defective in phytoene synthetase (445), a particulate enzyme (445 and references cited therein) (Fig. 9). Tracer experiments indicate a lesion between prephytoene pyrophosphate and phytoene (572). Alleles include those resulting in white and pale rose-white, e.g., 15300 and Y254MI65 (1042), and purple, e.g., MN58a (154). For complementation, see references 500 and 1041. Fine-structure mapping (500, 1042) needs reevaluation because of new information on the location of the arg-6 marker (797).	IR	B

Neurospora Crassa Wikipedia